Monthly Archives - February 2020

Hum Reprod Update. 2019 Nov 5;25(6) : 717-732. Doi: 10.1093/humupd/dmz029.

Wang R1,2,Li W2, Bordewijk EM3, LegroRS4, Zhang H5, Wu x6, Gao J6, Morin-Papunen L7, Homburg R8, Konig TE9, Moll E 10, Kar S11, Huang W12, Johnson NP1,13, Amer SA 14, Vegetti W15, Palomba S16, Falbo A17, Ozmen U18,Nazik H19, Williams CD20, Federica G21, Lord J22, Sahin Y23, Bhattacharya S24, Norman RJ1,25, Van Wely

Gayatri Mohanty1, Soumya Ranjan Jena1, Jasmine Nayak1, Sujata Kar2, Luna Samanta1

Am J Obstet Gynecol. 2017 Aug; 217(2):189.e1-189.e8.doi:10.1016/j.ajog.2017.04.007.Epub 2017 Apr 8.Am J Obstet Gynecol.

Chan JL 1, Kar S2, VenkyE3, Morin-Papunen L4, Piltonen T4, Puurunen J5, Tapanainen JS6, Maciel GAR7, Hayashida SAY7, Soares JM Jr7,Baracat EC7, Mellembakken JR8, Dokras A9.

Transient Receptor Potential Vanilloid sub-type 4 (TRPV4) is a non-selective cationic channel involved in regulation of temperature, osmolality and different ligand-dependent Ca(2+)-influx. Recently, we have demonstrated that TRPV4 is conserved in all vertebrates. Now we demonstrate that TRPV4 is endogenously expressed in all vertebrate sperm cells ranging from fish to mammals. In human sperm, TRPV4 is present as N-glycosylated protein and its activation induces Ca(2+)-influx. Its expression and localization differs in swim-up and swim-down cells suggesting that TRPV4 is an important determining factor for sperm motility. We demonstrate that pharmacological activation or inhibition of TRPV4 regulates Ca(2+)-wave propagation from head to tail. Such findings may have wide application in male fertility-infertility, contraception and conservation of endangered species as well.

Ashutosh Kumar^a, Rakesh Kumar Majhi^a,1, Nirlipta Swain^a,b,1, S.C.Giri^c, Sujata Kar^d, Luna Samanta^b, Chandan Goswami^a,*

Keywords: Calcium-wave; Evolution; Sperm; TRP channels; TRPV4; Vertebrates.

Comprehensive treatment of high risk maternal diseases like diabetes, hypertension, heart, kidney, liver disease , blood thrombophilias, sickle cell disease, RH isoimmunization and cancer survivors And Fetal congenital abnormalities, genetic diseases, twins, triplets with sophisticated testing such as Prenatal biochemical screens, amniocentesis, chorionic villus sampling, NIFTY (non invasive fetal trisomy), targeted fetal anomaly screening, high risk level three sonographies, fetal echocardiography.Lady Gynecologist Doctors in Bhubaneswar Odisha DR SUJATA KAR.

Histone retention, protein carbonylation, and lipid peroxidation in spermatozoa: Possible role in recurrent pregnancy loss

Contribution from a defective paternal genome has been attributed to be an important cause for spontaneous recurrent pregnancy loss (RPL). Increased oxidative stress results in decreased detoxification and is a cause for damage to chromatin, proteins, and membrane lipids. The present study aimed to explore if there is a significant relationship between retained histones due to defective packaging of DNA in spermatozoa and oxidative stress. RPL patients (n=16) with a history of ≥2 embryo losses before the 20th week of gestation and no female factor abnormality, and fertile healthy volunteers (n=20) as controls were included in the study. A significant difference in the levels of protein carbonylation and lipid peroxidation together with an increased retention of histones in the experimental groups was noticed. Histone carrying sites for oxidative modification such as arginine and lysine might be responsible for disturbing the paternal epigenomic control during early stages of embryonic differentiation leading to abortion.

Results and discussion

The semen was viscous and had a pH within the range of 7.0-8.0 in both the control and RPL groups. Routinely analyzed sperm attributes except sperm count and leukocyte concentration showed a distinct significant change in the RPL group (Table 1). The percentage of motile spermatozoa drastically dropped in the RPL group but it was still possible to achieve pregnancy as it is above the lower reference limit of 40% [WHO 2010]. However, the oxidative stress parameters of semen showed a significant increment in the RPL patient group

Gatatri Mohanty^a,*, Nirlipta Swain^a,b,*, Chandan Goswami^b, Sujata Kar^c , and Luna Samanta^a